Advances and Clinical Practice in Pyrosequencing by Guohua Zhou & Qinxin Song

Author:Guohua Zhou & Qinxin Song
Language: eng
Format: epub
Publisher: Springer New York, New York, NY

4.The cells were harvested by centrifugation (8000 × g for 3 min at 4 °C), then were resuspended in 40 mL 1× Binding buffer (0.5 mol/L NaCl, 20 mmol/L Tris–HCl, 5 mmol/L Imidazole, pH 7.9), added 1 μL lysozyme (100 g/L) into the buffer for 0.5 h at room temperature.

5.The cells were disrupted by gentle sonication on ice and centrifuged (10,000 × g for 30 min at 4 °C); the supernatants were purified by His•Bind Resin affinity chromatography. The purity of the protein preparations was assessed by SDS-PAGE.

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